Controlled dehydration of a ruthenium complex-DNA crystal induces reversible DNA kinking
Citation:
Hall,James P. J.P., Sánchez-Weatherby,Juan J., Alberti,Cora C., Quimper,Caroline Hurtado C.H., O'Sullivan,Kyra K., Brazier,John A. J.A., Winter,Graeme G., Sorensen,Thomas T., Kelly,John M. J.M., Cardin,David John D.J., Cardin,Christine J J C.J.J., Controlled dehydration of a ruthenium complex-DNA crystal induces reversible DNA kinking, Journal of the American Chemical Society, 136, 50, 2014, 17505-17512Download Item:
2Pub200 Hall Hydration JACS 2014 asap.pdf (PDF) 1.329Mb
Abstract:
Hydration-dependent DNA deformation has been known since Rosalind Franklin
recognised that the relative humidity of the sample had to be maintained to observe a single
conformation in DNA fibre diffraction. We now report for the first time the crystal structure, at the
atomic level, of a dehydrated form of a DNA duplex and demonstrate the reversible interconversion
to the hydrated form at room temperature. This system, containing d(TCGGCGCCGA) in the
presence of Λ-[Ru(TAP)2(dppz)]2+ (TAP = 1,4,5,8-tetraazaphenanthrene, dppz =
dipyridophenazine), undergoes a partial transition from an A/B hybrid to the A-DNA
conformation, at 84-79% relative humidity. This is accompanied by an increase in kink at the
central step from 22° to 51°, with a large movement of the terminal bases forming the intercalation
site. This transition is reversible on rehydration. Seven datasets, collected from one crystal at room
temperature, show the consequences of dehydration at near-atomic resolution. This result
highlights that crystals, traditionally thought of as static systems, are still dynamic and therefore
can be the subject of further experimentation.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
06/RF/CHP035
Author's Homepage:
http://people.tcd.ie/jmkellyDescription:
PUBLISHED
Author: KELLY, JOHN
Type of material:
Journal ArticleSeries/Report no:
Journal of the American Chemical Society136
50
Availability:
Full text availableKeywords:
Hydration-dependent DNA deformationDOI:
http://dx.doi.org/10.1021/ja508745xISSN:
00027863Licences: