Protein kinase C delta is a substrate of tissue transglutaminase and a novel autoantigen in coeliac disease
Item Type:Journal Article
Citation:Byrne G, Freeley M, Feighery C, Whelan A and Long A, Protein kinase C delta is a substrate of tissue transglutaminase and a novel autoantigen in coeliac disease, Clinical Immunology, 147, 1, 2013, 1 - 8
Post-translational modification of proteins by deamidation or transamidation by tissue transglutaminase (tTG) has been suggested as a possible mechanism for the development of autoimmunity. Sequence analysis of protein kinase C delta (PKCδ) identified an amino acid motif that suggested the possibility that PKCδ was a glutamine substrate of tTG and MALDI-TOF analysis of synthesised peptides from PKCδ proved that this was the case. Polymerisation experiments using recombinant tTG and biotinylated hexapeptide substrate incorporation assays demonstrated that PKCδ is a substrate for tTG-mediated transamidation. Elevated levels of anti-PKCδ antibodies were detected in sera from patients with coeliac disease (p<0.0001) but not from patients with other autoimmune disorders. These data suggest that a subset of patients with coeliac disease produce autoantibodies against PKCδ and that this response may stem from a tTG-PKCδ substrate interaction.
Author: FREELEY, MICHAEL
Type of material:Journal Article
Series/Report no:Clinical Immunology
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