dc.contributor.author | MILLS, KINGSTON | en |
dc.date.accessioned | 2015-01-09T11:39:05Z | |
dc.date.available | 2015-01-09T11:39:05Z | |
dc.date.issued | 2013 | en |
dc.date.submitted | 2013 | en |
dc.identifier.citation | Morine, M.J., Toomey, S., McGillicuddy, F.C., Reynolds, C.M., Power, K.A., Browne, J.A., Loscher, C., (...), Roche, H.M., Network analysis of adipose tissue gene expression highlights altered metabolic and regulatory transcriptomic activity in high-fat-diet-fed IL-1RI knockout mice, Journal of Nutritional Biochemistry, 24, 5, 2013, 788-795 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/72967 | |
dc.description | PUBLISHED | en |
dc.description.abstract | A subacute inflammatory phenotype is implicated in the pathology of insulin resistance (IR) and type 2 diabetes mellitus. Interleukin (IL)-1α and IL-1β are produced by innate immune cells, including macrophages, and mediate their inflammatory response through the IL-1 type I receptor (IL-IRI). This study sought to understand the transcriptomic signature of adipose tissue in obese IL-1RI−/− mice. Following dietary intervention, markers of insulin sensitivity and inflammation in adipose tissue were determined, and gene expression was assessed with microarrays. IL-1RI−/− mice fed a high-fat diet (HFD) had significantly lower plasma inflammatory cytokine concentrations than wild-type mice. Metabolic network analysis of transcriptomic effects identified up-regulation and co-expression of genes involved in lipolysis, lipogenesis and tricarboxylic acid (TCA) cycle. Further assessment of gene expression in a network of protein interactions related to innate immunity highlighted Stat3 as a potential transcriptional regulator of IL-1 signalling. The complex, downstream effects of IL-1 signalling through the IL-1RI receptor remain poorly defined. Using network-based analyses of transcriptomic signatures in IL-1RI−/− mice, we have identified expression changes in genes involved in lipid cycling and TCA cycle, which may be more broadly indicative of a restoration of mitochondrial function in the context of HFD. Our results also highlight a potential role for Stat3 in linking IL-1 signalling to adipogenesis and IR. | en |
dc.format.extent | 788-795 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Journal of Nutritional Biochemistry | en |
dc.relation.ispartofseries | 24 | en |
dc.relation.ispartofseries | 5 | en |
dc.rights | Y | en |
dc.subject | Adipose tissue | en |
dc.subject | Insulin resistance | en |
dc.subject | Network analysis | en |
dc.subject | Transcriptomic profiling | en |
dc.subject | IL-1 receptor type I | en |
dc.subject | Inflammation | en |
dc.title | Network analysis of adipose tissue gene expression highlights altered metabolic and regulatory transcriptomic activity in high-fat-diet-fed IL-1RI knockout mice | en |
dc.type | Journal Article | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsor | Irish Research Council for Science and Engineering Technology (IRCSET) | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/millsk | en |
dc.identifier.rssinternalid | 87014 | en |
dc.identifier.doi | http://dx.doi.org/10.1016/j.jnutbio.2012.04.012 | en |
dc.rights.ecaccessrights | openAccess | |
dc.contributor.sponsorGrantNumber | 11/PI/1119 | en |
dc.contributor.sponsorGrantNumber | (06/IM.1/B105; | en |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |