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dc.contributor.authorHODKINSON, TREVORen
dc.date.accessioned2014-12-16T13:32:14Z
dc.date.available2014-12-16T13:32:14Z
dc.date.issued2013en
dc.date.submitted2013en
dc.identifier.citationPerdereau AC, Douglas GC, Hodkinson TR, Kelleher CT, High levels of variation in Salix lignocellulose genes revealed using poplar genomic resources, Biotechnology for Biofuels, 6, 2013, 1 - 15en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/72492
dc.descriptionPUBLISHEDen
dc.description.abstractBackground Little is known about the levels of variation in lignin or other wood related genes in Salix, a genus that is being increasingly used for biomass and biofuel production. The lignin biosynthesis pathway is well characterized in a number of species, including the model tree Populus. We aimed to transfer the genomic resources already available in Populus to its sister genus Salix to assess levels of variation within genes involved in wood formation. Results Amplification trials for 27 gene regions were undertaken in 40 Salix taxa. Twelve of these regions were sequenced. Alignment searches of the resulting sequences against reference databases, combined with phylogenetic analyses, showed the close similarity of these Salix sequences to Populus, confirming homology of the primer regions and indicating a high level of conservation within the wood formation genes. However, all sequences were found to vary considerably among Salix species, mainly as SNPs with a smaller number of insertions-deletions. Between 25 and 176 SNPs per kbp per gene region (in predicted exons) were discovered within Salix. Conclusions The variation found is sizeable but not unexpected as it is based on interspecific and not intraspecific comparison; it is comparable to interspecific variation in Populus. The characterisation of genetic variation is a key process in pre-breeding and for the conservation and exploitation of genetic resources in Salix. This study characterises the variation in several lignocellulose gene markers for such purposes.en
dc.format.extent1en
dc.format.extent15en
dc.language.isoenen
dc.relation.ispartofseriesBiotechnology for Biofuelsen
dc.relation.ispartofseries6en
dc.rightsYen
dc.subjectCloningen
dc.subjectSNPsen
dc.subjectPopulusen
dc.subjectLigninen
dc.subjectDiversityen
dc.subjectCelluloseen
dc.titleHigh levels of variation in Salix lignocellulose genes revealed using poplar genomic resourcesen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/hodkinsten
dc.identifier.rssinternalid88119en
dc.identifier.doihttp://dx.doi.org/doi:10.1186/1754-6834-6-114en
dc.rights.ecaccessrightsopenAccess
dc.subject.TCDThemeGenes & Societyen
dc.subject.TCDThemeSmart & Sustainable Planeten
dc.identifier.rssurihttp://www.biotechnologyforbiofuels.com/content/6/1/114en


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