Strategies for the purification of membrane proteins
Item Type:Book Chapter
Citation:Strategies for the purification of membrane proteins, Dermot Walls & Sinead Loughran, Methods in Molecular Biology, Springer, 2011, 485 - 496, Smith SM
159928_1_En_29_Chapter_OnlinePDF..pdf (PDF) 262.7Kb
Although membrane proteins account for 20–30% of the coding regions of all sequenced genomes and play crucial roles in many fundamental cell processes, there are relatively few membranes with known 3D structure. This is likely due to technical challenges associated with membrane protein extraction, solubili - sation, and purification. Membrane proteins are classified based on the level of interaction with membrane lipid bilayers, with peripheral membrane proteins associating non-covalently with the membrane, and integral membrane proteins associating more strongly by means of hydrophobic interactions. Generally speaking, peripheral membrane proteins can be purified by milder techniques than integral membrane proteins, whose extraction requires phospholipid bilayer disruption by detergents. Here, important criteria for strategies of membrane protein purification are addressed, with a focus on the initial stages of membrane protein solublilisation, where problems are most frequently encountered. Protocols are outlined for the successful extraction of peripheral membrane proteins, solubilisation of integral membrane proteins, and detergent removal which is important not only for retaining native protein stability and biological func - tions, but also for the efficiency of later purification techniques
Author: SMITH, SINEAD
Other Titles:Methods in Molecular Biology
Type of material:Book Chapter
Availability:Full text available
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