dc.contributor.author | DORMAN, CHARLES | en |
dc.date.accessioned | 2014-09-23T11:02:49Z | |
dc.date.available | 2014-09-23T11:02:49Z | |
dc.date.issued | 2003 | en |
dc.date.submitted | 2003 | en |
dc.identifier.citation | McKenna S, Beloin C, Dorman CJ, In vitro DNA binding properties of VirB, the Shigella flexneri virulence regulatory protein, FEBS Letters, 545, 2-3, 2003, 183 - 187 | en |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/71360 | |
dc.description | PUBLISHED | en |
dc.description.abstract | The DNA-binding activity of the Shigella flexneri VirB transcription factor was studied in vitro. The protein was found to bind non-specifically to DNA, but showed preferential binding to VirB-dependent promoter sequences. DNA binding was contingent on the presence of an intact helix-turn-helix motif. While high molecular mass protein–DNA complexes were formed in both specific and non-specific interactions with DNA, mutant derivatives of VirB lacking a leucine zipper domain or a carboxyl-terminal-located oligomerisation domain formed discrete complexes, indicating that an ability to oligomerise on DNA was responsible for the formation of high molecular mass complexes by the wild-type protein. | en |
dc.format.extent | 183 | en |
dc.format.extent | 187 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | FEBS Letters | en |
dc.relation.ispartofseries | 545 | en |
dc.relation.ispartofseries | 2-3 | en |
dc.rights | Y | en |
dc.subject | Shigella flexneri | en |
dc.subject | Helix-turn-helix motif; | en |
dc.subject | Leucine zipper | en |
dc.subject | Gene regulation | en |
dc.subject | Transcription facto | en |
dc.subject | VirB protein | en |
dc.title | In vitro DNA binding properties of VirB, the Shigella flexneri virulence regulatory protein | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/cjdorman | en |
dc.identifier.rssinternalid | 3495 | en |
dc.identifier.doi | http://dx.doi.org/10.1016/S0014-5793(03)00524-6 | en |
dc.rights.ecaccessrights | openAccess | |
dc.subject.TCDTheme | Genes & Society | en |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.identifier.rssuri | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-48MYG9N-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=bb1e863ed15a282a7c024a963011ab11 | en |