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dc.contributor.authorCAFFREY, MARTINen
dc.date.accessioned2014-06-26T11:34:59Z
dc.date.available2014-06-26T11:34:59Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationLi, DF, Lee, J, Caffrey, M, Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen, CRYSTAL GROWTH & DESIGN, 11, 2, 2011, 530-537en
dc.identifier.issn1528-7483en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/69874
dc.descriptionPUBLISHEDen
dc.description.abstractThe default lipid for the bulk of the crystallogenesis studies performed to date using the cubic mesophase method is monoolein. There is no good reason however, why this 18-carbon, cis - monounsaturated monoacylglycerol should be the preferred lipid for all target membrane proteins. The latter come from an array of biomembrane types with varying properties that include hydrophobic thickness, intrinsic curvature, lateral pressure profile, lipid and protein makeup, and compositional asymmetry. Thus, it seems reasonable that screening for crystallizability based on the identity of the lipid creating the hosting mesophase would be worthwhile. For this, monoacylglycerols with differing acyl chain characteristics, such as length and olefinic bond position, must be available. A lipid synthesis and purification program is in place in the author's laboratory to serve this need. In the current study with the outer membrane sugar transporter, OprB, we demonstrate the utility of host lipid screening as a means for generating diffraction- quality crystals. Host lipid screening is likely to prove a generally useful strategy for mesophase- based crystallization of membrane proteinsen
dc.format.extent530-537en
dc.language.isoenen
dc.relation.ispartofseriesCRYSTAL GROWTH & DESIGNen
dc.relation.ispartofseries11en
dc.relation.ispartofseries2en
dc.rightsYen
dc.subjectmembrane protein structure;en
dc.subjectOprB;en
dc.subjectouter membrane proteins;en
dc.subjectporinen
dc.subjectscreenen
dc.subjectsugar transporteren
dc.subjectX- ray crystallographyen
dc.titleCrystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screenen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mcaffreen
dc.identifier.rssinternalid94987en
dc.identifier.doihttp://dx.doi.org/10.1021/cg101378sen
dc.rights.ecaccessrightsopenAccess


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