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dc.contributor.authorCOLEMAN, DAVID
dc.contributor.authorMORAN, GARY
dc.contributor.authorSULLIVAN, DEREK
dc.contributor.authorCOLEMAN, DAVIDen
dc.contributor.authorMORAN, GARYen
dc.date.accessioned2014-05-19T14:52:57Z
dc.date.available2014-05-19T14:52:57Z
dc.date.issued2007-09
dc.date.issued2007en
dc.date.submitted2007en
dc.identifier47217en
dc.identifier.citationMORAN GP, MacCALLUM DM, SPIERING MJ, COLEMAN DC, SULLIVAN DJ., DIFFERENTIAL REGULATION OF THE TRANSCRIPTIONAL REPRESSOR NRG1 ACCOUNTS FOR ALTERED HOST CELL INTERACTIONS IN CANDIDA ALBICANS AND CANDIDA DUBLINIENSIS, MOLECULAR MICROBIOLOGY, 66, 4, 2007, 915, 929en
dc.identifier.otherY
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/69261
dc.descriptionPUBLISHEDen
dc.description.abstractCandida dubliniensis is genetically closely related to C. albicans, but causes fewer infections in humans and exhibits reduced virulence and filamentation in animal models of infection. We investigated the role of the C. dubliniensis transcriptional repressor-encoding gene CdNRG1 in regulating this phenotype. Deletion of both copies of CdNRG1 increased the formation of true hyphae by C. dubliniensis in response to serum, exogenous cAMP and CO2. In addition, deletion of CdNRG1 greatly enhanced filamentation and survival of C. dubliniensis in co-culture with murine macrophages. In the reconstituted human oral epithelium (RHE) infection model, the nrg1? mutant caused increased tissue damage relative to the wild-type strain. However, deletion of CdNRG1 did not change the virulence of C. dubliniensis in the systemic mouse model of infection. The increased rate of hypha formation in C. albicans relative to C. dubliniensis in response to phagocytosis by macrophages and serum was associated with rapid downregulation of NRG1 expression in C. albicans. This study demonstrates that the reduced virulence of C. dubliniensis is due to the inability of this species to modulate NRG1 expression in response to the same environmental signals that promote filamentation in C. albicans.en
dc.format.extent915en
dc.format.extent929en
dc.language.isoenen
dc.publisherBlackwell Publishingen
dc.relation.ispartofseries66en
dc.relation.ispartofseries4en
dc.relation.ispartofseriesMOLECULAR MICROBIOLOGYen
dc.rightsYen
dc.subjectCandidaen
dc.subjectdubliniensisen
dc.subjectvirulenceen
dc.subjecthyphaeen
dc.titleDIFFERENTIAL REGULATION OF THE TRANSCRIPTIONAL REPRESSOR NRG1 ACCOUNTS FOR ALTERED HOST CELL INTERACTIONS IN CANDIDA ALBICANS AND CANDIDA DUBLINIENSISen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/gmoran
dc.identifier.peoplefinderurlhttp://people.tcd.ie/dcolemanen
dc.identifier.rssinternalid47217en
dc.identifier.rssurihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17927699&dopt=Abstracten


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