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dc.contributor.authorVoorheis, Howarden
dc.contributor.authorFallon, Padraicen
dc.contributor.authorPorter, Richarden
dc.contributor.authorNolan, Dereken
dc.date.accessioned2014-04-11T09:25:47Z
dc.date.available2014-04-11T09:25:47Z
dc.date.issued2008en
dc.date.submitted2008en
dc.identifier.citationAlison E. Adams, Orla Hanrahan, Derek N. Nolan, H. Paul Voorheis, Padraic Fallon and Richard K. Porter, Images of mitochondrial UCP 1 in mouse thymocytes using confocal microscopy, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1777, 2, 2008, 115 - 117en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/68465
dc.descriptionPUBLISHEDen
dc.descriptionPMID: 17996719en
dc.description.abstractThe aim of this study was to demonstrate the constitutive expression of mitochondrial uncoupling protein 1 (UCP 1) in pure thymocytes using laser scanning confocal microscopic imagery. To that end we probed thymocytes from UCP 1 knock-out and wild-type mice. Mitochondrial location in thymocytes was determined using Mitotracker Red and the nucleus was labelled using Hoescht stain. We demonstrate that all cells investigated were thymocytes as determined by a monoclonal antibody specific for the thymocyte surface marker Thy 1 (CD90) pre-coupled to a fluorescent labelled (Alexa 448, green). Using a primary peptide antibody specific to UCP 1, and secondary fluorescently labelled (Alexa 647, magenta) antibody, we were able to demonstrate that UCP 1 is associated with mitochondria in thymocytes from UCP 1 wild-type mice but not thymocytes from UCP1-knock-out mice. These are the first images demonstrating the presence of UCP 1 in thymocyte mitochondria, in situ, and the first to clearly demonstrate UCP 1 expression in cells other than brown adipocytes. We conclude that mouse thymocytes contain UCP 1 in their mitochondria.en
dc.format.extent115en
dc.format.extent117en
dc.language.isoenen
dc.relation.ispartofseriesBiochimica et Biophysica Acta (BBA) - Bioenergeticsen
dc.relation.ispartofseries1777en
dc.relation.ispartofseries2en
dc.rightsYen
dc.subjectUncoupling protein-1en
dc.subjectThymusen
dc.subjectThymocytesen
dc.subjectMitochondriaen
dc.subjectUCP 1 knock-out miceen
dc.subjectConfocal microscopyen
dc.titleImages of mitochondrial UCP 1 in mouse thymocytes using confocal microscopyen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/denolanen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/rkporteren
dc.identifier.peoplefinderurlhttp://people.tcd.ie/hvoorhisen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pfallonen
dc.identifier.rssinternalid63114en
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbabio.2007.10.003en
dc.rights.ecaccessrightsOpenAccess
dc.subject.TCDThemeGenes & Societyen
dc.identifier.orcid_id0000-0002-3742-4304en


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