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dc.contributor.authorCREAGH, EMMA
dc.contributor.authorMARTIN, SEAMUS JOSEPH
dc.date.accessioned2012-01-23T10:22:55Z
dc.date.available2012-01-23T10:22:55Z
dc.date.issued2001
dc.date.submitted2001en
dc.identifier.citationAdrain C, Creagh EM, Martin SJ, Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2, THE EMBO JOURNAL, 20, 2001, 6627 - 6636en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/61798
dc.descriptionPUBLISHEDen
dc.description.abstractSmac/DIABLO is a mitochondrial protein that potentiates some forms of apoptosis, possibly by neutralizing one or more members of the IAP family of apoptosis inhibitory proteins. Smac has been shown to exit mitochondria and enter the cytosol during apoptosis triggered by UV- or ?-irradiation. Here, we report that Smac/DIABLO export from mitochondria into the cytosol is provoked by cytotoxic drugs and DNA damage, as well as by ligation of the CD95 death receptor. Mitochondrial efflux of Smac/DIABLO, in response to a variety of pro-apoptotic agents, was profoundly inhibited in Bcl-2-overexpressing cells. Thus, in addition to modulating apoptosis-associated mitochondrial cytochrome c release, Bcl-2 also regulates Smac release, suggesting that both molecules may escape via the same route. However, whereas cell stress-associated mitochondrial cytochrome c release was largely caspase independent, release of Smac/DIABLO in response to the same stimuli was blocked by a broad-spectrum caspase inhibitor. This suggests that apoptosis-associated cytochrome c and Smac/DIABLO release from mitochondria do not occur via the same mechanism. Rather, Smac/DIABLO efflux from mitochondria is a caspase-catalysed event that occurs downstream of cytochrome c release.en
dc.format.extent6627en
dc.format.extent6636en
dc.language.isoenen
dc.relation.ispartofseriesTHE EMBO JOURNAL;
dc.relation.ispartofseries20;
dc.rightsYen
dc.subjectBiochemistryen
dc.subjectmitochondrial proteinsen
dc.titleApoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/martinsj
dc.identifier.peoplefinderurlhttp://people.tcd.ie/ecreagh
dc.identifier.rssinternalid5064
dc.identifier.rssurihttp://dx.crossref.org/10.1093%2Femboj%2F20.23.6627en


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