The University of Dublin | Trinity College -- Ollscoil Átha Cliath | Coláiste na Tríonóide
Trinity's Access to Research Archive
Home :: Log In :: Submit :: Alerts ::

TARA >
Vice Deanery of Genetics & Microbiology >
Microbiology >
Microbiology (Scholarly Publications) >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2262/61058

Title: The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p
Author: BOND, URSULA MARY
JAMES, THARAPPEL C
Sponsor: 
Name Grant Number
06/RFP/GEN/060

Author's Homepage: http://people.tcd.ie/ubond
http://people.tcd.ie/jthrppel
Keywords: Genetics
mRNAs
Issue Date: 2012
Citation: Suzanne Beggs, Tharappel C. James and Ursula Bond, The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p, Nucleic Acids Research, 40, 6, 2012, 2700 - 2711
Series/Report no.: Nucleic Acids Research
40
6
Abstract: Yeast histone mRNAs are polyadenylated, yet factors such as Rrp6p and Trf4p, required for the 3′-end processing of non-polyadenylated RNAs, contribute to the cell cycle regulation of these transcripts. Here, we investigated the role of other known 3′-end processing/transcription termination factors of non-polyadenylated RNA in the biogenesis of histone mRNAs, specifically the Nab3p/Nrd1p/Sen1p complex. We also re-evaluated the polyadenylation status of these mRNAs during the cell cycle. Our analysis reveals that yeast histone mRNAs have shorter than average PolyA tails and the length of the PolyA tail varies during the cell cycle; S-phase histone mRNAs possess very short PolyA tails while in G1, the tail length is relatively longer. Inactivation of either Sen1p or Rrp6p leads to a decrease in the PolyA tail length of histone mRNAs. Our data also show that Sen1p contributes to 3′-end processing of histone primary transcripts. Thus, histone mRNAs are distinct from the general pool of yeast mRNAs and 3′-end processing and polyadenylation contribute to the cell cycle regulation of these transcripts.
Description: PUBLISHED
URI: http://hdl.handle.net/2262/61058
Related links: http://dx.doi.org/10.1093/nar/gkr1108
Appears in Collections:Microbiology (Scholarly Publications)

Files in This Item:

File Description SizeFormat
nar.gkr1108.full.pdfPublished (publisher's copy) - Peer Reviewed3.42 MBAdobe PDFView/Open


This item is protected by original copyright


Please note: There is a known bug in some browsers that causes an error when a user tries to view large pdf file within the browser window. If you receive the message "The file is damaged and could not be repaired", please try one of the solutions linked below based on the browser you are using.

Items in TARA are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback