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dc.contributor.authorAMU, SYLVIE
dc.date.accessioned2011-09-26T14:55:08Z
dc.date.available2011-09-26T14:55:08Z
dc.date.issued2011
dc.date.submitted2011en
dc.identifier.citationSylvie Amu and Mikael Brisslert, Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood, Clinical and Developmental Immunology, 2011, 481948, 2011en
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/59668
dc.descriptionPUBLISHEDen
dc.description.abstractBackground. We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood. Material and Methods. Mononuclear cell fraction from human cord blood ( ? = 3 4 ) and peripheral adult blood ( ? = 2 2 ) was sorted into CD20+CD25+ and CD20+CD25- B-cell populations. Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion. Results. CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20+ B cells. They are characterised by high expression of CD5 in cord blood and CD27 in adult blood. CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass. Conclusions. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action.ncen
dc.language.isoenen
dc.relation.ispartofseriesClinical and Developmental Immunology;
dc.relation.ispartofseries2011;
dc.relation.ispartofseries481948;
dc.rightsYen
dc.subjectImmunologyen
dc.subjectInfectionen
dc.subjectB-cellsen
dc.titlePhenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blooden
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/amus
dc.identifier.rssinternalid75095
dc.identifier.rssurihttp://dx.doi.org/10.1155/2011/481948en


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