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dc.contributor.authorKelly, Danielen
dc.contributor.authorHoey, Daviden
dc.date.accessioned2011-09-16T14:06:21Z
dc.date.available2011-09-16T14:06:21Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationHoey, D.A., Kelly, D.J., Jacobs, C.R., A role for the primary cilium in paracrine signaling between mechanically stimulated osteocytes and mesenchymal stem cells., Biochemical and Biophysical Research Communications, 412, 1, 2011, 182-187en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/59504
dc.descriptionPUBLISHEDen
dc.description.abstractBone turnover is a mechanically regulated process, coordinated in part by the network of mechanosensitive osteocytes residing within the tissue. The recruitment and bone forming activity of the mesenchymal derived osteoblast is determined by numerous factors including mechanical loading. It is therefore somewhat surprising that although mechanically regulated signaling between the coordinating osteocytes and mesenchymal stem cells (MSCs) should exist, to date it has not been directly demonstrated. In this study, conditioned media from mechanically stimulated osteocytes (MLO-Y4 cell line) was collected and added to MSCs (C3H10T1/2 cell line). The addition of mechanically stimulated osteocyte conditioned media resulted in a significant upregulation of the osteogenic genes OPN and COX-2 in MSCs compared to statically cultured conditioned media, demonstrating a novel paracrine signaling mechanism between the two cell types. The same mechanically conditioned media did not alter gene expression in osteoblasts (MC3T3 cell line), and mechanically stimulated osteoblast conditioned media did not alter gene expression in MSCs demonstrating that this signaling is unique to osteocytes and MSCs. Finally, the upregulation in osteogenic genes in MSCs was not observed if primary cilia formation was inhibited prior to mechanical stimulation of the osteocyte. In summary, the results of this study indicate that soluble factors secreted by osteocytes in response to mechanical stimulation can enhance osteogenic gene expression in MSCs demonstrating a novel, unique signaling mechanism and introduces a role for the primary cilium in flow mediated paracrine signaling in bone thereby highlighting the cilium as a potential target for therapeutics aimed at enhancing bone formation.en
dc.description.sponsorshipFunding provided by the Fulbright Program, Science Foundation Ireland (08/Y15/B1336), an IRCSET-Marie Curie International Mobility Fellowship in Science, Engineering and Technology, New York State Stem Cell Grant (N089-210) and NIH Grants (AR45989 and AR54156).en
dc.format.extent182-187en
dc.language.isoenen
dc.relation.ispartofseriesBiochemical and Biophysical Research Communicationsen
dc.relation.ispartofseries412en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectBioengineeringen
dc.subjectOsteocyteen
dc.subjectMesenchymal stem cellen
dc.titleA role for the primary cilium in paracrine signaling between mechanically stimulated osteocytes and mesenchymal stem cells.en
dc.typeJournal Articleen
dc.contributor.sponsorMarie Curieen
dc.contributor.sponsorNational Institutes of Health (NIH)en
dc.contributor.sponsorNational Institutes of Health (NIH)en
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.contributor.sponsorIrish Research Council for Science and Engineering Technology (IRCSET)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kellyd9en
dc.identifier.peoplefinderurlhttp://people.tcd.ie/dahoeyen
dc.identifier.rssinternalid74334en
dc.contributor.sponsorGrantNumberN089-210en
dc.contributor.sponsorGrantNumberAR54156en
dc.contributor.sponsorGrantNumberAR45989en
dc.contributor.sponsorGrantNumber08/Y15/B133en
dc.subject.TCDThemeNext Generation Medical Devicesen
dc.identifier.rssurihttp://dx.doi.org/10.1016/j.bbrc.2011.07.072en
dc.identifier.orcid_id0000-0003-4091-0992en


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