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dc.contributor.authorPorter, Richarden
dc.date.accessioned2011-09-02T15:05:16Z
dc.date.available2011-09-02T15:05:16Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationOrlagh M. Kelly, Richard K. Porter, Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1807, 9, 2011, 1064-1074en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/59261
dc.descriptionPUBLISHEDen
dc.description.abstractMitochondrial uncoupling protein 3 (UCP3) is consituitively expressed in mitochondria from thymus and spleen of mice, and confocal microscopy has been used to visualize UCP3 in situ in mouse thymocytes. UCP3 is present decrease by a half in thymus and a fifth in spleen after three weeks, probably reflecting the suckling to weaning transition. UCP3 protein levels increase ~3-fold in thymus on starvation, but expression levels in spleen were unaffected by starvation. Lack of UCP3 had little effect on thymus mass or thymocyte number . However, lack of UCP3 affected spleen mass and splenocyte number (in the fasted state) and results in reduced CD4+ single positive cell numbers and reduced double negative cells in the thymus, but as a 2-fold increase in the proportion of CD4 + , CD8 + and DP cells in spleen. Starvation attenuates these proportionate differences in the spleen. A lack of UCP3 had no apparent effect on basal oxygen consumption of thymocytes or splenocytes or on oxygen consumption due to mitochondrial proton leak. Splenocytes from UCP3 knock-out mice are also more resistant to apoptosis than those from wild-type mice. Overall we can conclude that UCP3 affects thymocyte and spleen cell profiles in the fed and fasted state.en
dc.description.sponsorshipFunding for this study was provided by a Trinity College Studentship stipend to O. Kelly, a Health Research Board project grant (RP/2008/61) and Science Foundation Ireland Principal Investigator award (SFI 06/IN.1/B67). R.K. Porter is a member of the MITOFOOD COST Action (FA0602).en
dc.format.extent1064-1074en
dc.language.isoenen
dc.relation.ispartofseriesBiochimica et Biophysica Acta (BBA) - Bioenergeticsen
dc.relation.ispartofseries1807en
dc.relation.ispartofseries9en
dc.rightsYen
dc.subjectBiochemistryen
dc.subjectMitochondrial uncoupling protein 3 (UCP3)en
dc.titleAbsence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted miceen
dc.typeJournal Articleen
dc.contributor.sponsorHealth Research Board (HRB)en
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/rkporteren
dc.identifier.rssinternalid73619en
dc.contributor.sponsorGrantNumberRP/2008/61en
dc.contributor.sponsorGrantNumber06/IN.1/B67en
dc.subject.TCDThemeGenes & Societyen
dc.identifier.rssurihttps://www.ncbi.nlm.nih.gov/pubmed/21689632en


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