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dc.contributor.authorULRICH, DANIELen
dc.contributor.authorROWAN, MICHAELen
dc.date.accessioned2011-08-24T14:17:21Z
dc.date.available2011-08-24T14:17:21Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationLanté F, Toledo-Salas JC, Ondrejcak T, Rowan MJ, Ulrich D, Removal of Synaptic Ca2+-Permeable AMPA Receptors during Sleep., The Journal of Neuroscience, 31, 11, 2011, 3953-61en
dc.identifier.issn0270-6474en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/59033
dc.descriptionPUBLISHEDen
dc.description.abstracthere is accumulating evidence that sleep contributes to memory formation and learning, but the underlying cellular mechanisms are incompletely understood. To investigate the impact of sleep on excitatory synaptic transmission, we obtained whole-cell patch-clamp recordings from layer V pyramidal neurons in acute slices of somatosensory cortex of juvenile rats (postnatal days 21-25). In animals after the dark period, philanthotoxin 74 (PhTx)-sensitive calcium-permeable AMPA receptors (CP-AMPARs) accounted for ~25% of total EPSP size, and current-voltage (I-V) relationships of the underlying EPSCs showed inward rectification. In contrast, in similar experiments after the light period, EPSPs were PhTx insensitive with linear I-V characteristics, indicating that CP-AMPARs were less abundant. Combined EEG and EMG recordings confirmed that slow-wave sleep-associated delta wave power peaked at the onset of the more quiescent, lights-on phase of the cycle. Subsequently, we show that burst firing, a characteristic action potential discharge mode of layer V pyramidal neurons during slow-wave sleep has a dual impact on synaptic AMPA receptor composition: repetitive burst firing without synaptic stimulation eliminated CP-AMPARs by activating serine/threonine phosphatases. Additionally, repetitive burst-firing paired with EPSPs led to input-specific long-term depression (LTD), affecting Ca(2+) impermeable AMPARs via protein kinase C signaling. In agreement with two parallel mechanisms, simple bursts were ineffective after the light period but paired bursts induced robust LTD. In contrast, incremental LTD was generated by both conditioning protocols after the dark cycle. Together, our results demonstrate qualitative changes at neocortical glutamatergic synapses that can be induced by discharge patterns characteristic of non-rapid eye movement sleep.en
dc.format.extent3953-61en
dc.language.isoenen
dc.relation.ispartofseriesThe Journal of Neuroscienceen
dc.relation.ispartofseries31en
dc.relation.ispartofseries11en
dc.rightsYen
dc.subjectNeuroscienceen
dc.subjectSleepen
dc.subjectMemory functionen
dc.titleRemoval of Synaptic Ca2+-Permeable AMPA Receptors during Sleep.en
dc.typeJournal Articleen
dc.contributor.sponsorHealth Research Board (HRB)en
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/ulrichden
dc.identifier.peoplefinderurlhttp://people.tcd.ie/mrowanen
dc.identifier.rssinternalid72716en
dc.identifier.doihttp://dx.doi.org/10.1523/JNEUROSCI.3210-10.2011en
dc.contributor.sponsorGrantNumberRP/2008/98en
dc.contributor.sponsorGrantNumber06/IN.1/B88en
dc.subject.TCDThemeNeuroscienceen
dc.identifier.rssurihttp://dx.doi.org/10.1523/JNEUROSCI.3210-10.2011en


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