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dc.contributor.authorBRADLEY, DANIELen
dc.date.accessioned2011-05-24T13:56:06Z
dc.date.available2011-05-24T13:56:06Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationNoyes H, Brass A, Obara I, Anderson S, Archibald AL, Bradley DG, Fisher P, Freeman A, Gibson J, Gicheru M, Hall L, Hanotte O, Hulme H, McKeever D, Murray C, Oh SJ, Tate C, Smith K, Tapio M, Wambugu J, Williams DJ, Agaba M, Kemp SJ, Genetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection., Proceedings of the National Academy of Sciences of the United States of America, 108, 22, 2011, 9304-9en
dc.identifier.issn0027-8424en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/55980
dc.descriptionPUBLISHEDen
dc.description.abstractAfrican bovine trypanosomiasis caused by Trypanosoma sp., is a major constraint on cattle productivity in sub-Saharan Africa. Some African Bos taurus breeds are highly tolerant of infection, but the potentially more productive Bos indicus zebu breeds are much more susceptible. Zebu cattle are well adapted for plowing and haulage, and increasing their tolerance of trypanosomiasis could have a major impact on crop cultivation as well as dairy and beef production. We used three strategies to obtain short lists of candidate genes within QTL that were previously shown to regulate response to infection. We analyzed the transcriptomes of trypanotolerant N'Dama and susceptible Boran cattle after infection with Trypanosoma congolense. We sequenced EST libraries from these two breeds to identify polymorphisms that might underlie previously identified quantitative trait loci (QTL), and we assessed QTL regions and candidate loci for evidence of selective sweeps. The scan of the EST sequences identified a previously undescribed polymorphism in ARHGAP15 in the Bta2 trypanotolerance QTL. The polymorphism affects gene function in vitro and could contribute to the observed differences in expression of the MAPK pathway in vivo. The expression data showed that TLR and MAPK pathways responded to infection, and the former contained TICAM1, which is within a QTL on Bta7. Genetic analyses showed that selective sweeps had occurred at TICAM1 and ARHGAP15 loci in African taurine cattle, making them strong candidates for the genes underlying the QTL. Candidate QTL genes were identified in other QTL by their expression profile and the pathways in which they participate.en
dc.format.extent9304-9en
dc.language.isoenen
dc.relation.ispartofseriesProceedings of the National Academy of Sciences of the United States of Americaen
dc.relation.ispartofseries108en
dc.relation.ispartofseries22en
dc.rightsYen
dc.subjectGeneticsen
dc.subjectImmunologyen
dc.subjectbovine trypanosomiasisen
dc.titleGenetic and expression analysis of cattle identifies candidate genes in pathways responding to Trypanosoma congolense infection.en
dc.typeJournal Articleen
dc.contributor.sponsorWellcome Trusten
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/dbradleyen
dc.identifier.rssinternalid73399en
dc.identifier.doihttp://dx.doi.org/10.1073/pnas.1013486108en
dc.contributor.sponsorGrantNumberGR066764MAen
dc.contributor.sponsorGrantNumber02-IN.1-B256en
dc.subject.TCDThemeGenes & Societyen
dc.identifier.rssurihttp://dx.doi.org/10.1073/pnas.1013486108en


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