Show simple item record

dc.contributor.authorFINN, STEPHENen
dc.date.accessioned2011-03-22T15:15:28Z
dc.date.available2011-03-22T15:15:28Z
dc.date.issued2010en
dc.date.submitted2010en
dc.identifier.citationNguyen PL, Ma J, Chavarro JE, Freedman ML, Lis R, Fedele G, Fiore C, Qiu W, Fiorentino M, Finn S, Penney KL, Eisenstein A, Schumacher FR, Mucci LA, Stampfer MJ, Giovannucci E, Loda M, Fatty acid synthase polymorphisms, tumor expression, body mass index, prostate cancer risk, and survival., Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 28, 25, 2010, 3958-64en
dc.identifier.issn0732-183Xen
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/53770
dc.descriptionPUBLISHEDen
dc.description.abstractBACKGROUND: Overexpression of the fatty acid synthase (FASN) gene has been implicated in prostate carcinogenesis. We sought to directly assess the oncogenic potential of FASN. METHODS: We used immortalized human prostate epithelial cells (iPrECs), androgen receptor-overexpressing iPrECs (AR-iPrEC), and human prostate adenocarcinoma LNCaP cells that stably overexpressed FASN for cell proliferation assays, soft agar assays, and tests of tumor formation in immunodeficient mice. Transgenic mice expressing FASN in the prostate were generated to assess the effects of FASN on prostate histology. Apoptosis was evaluated by Hoechst 33342 staining and by fluorescence-activated cell sorting in iPrEC-FASN cells treated with stimulators of the intrinsic and extrinsic pathways of apoptosis (ie, camptothecin and anti-Fas antibody, respectively) or with a small interfering RNA (siRNA) targeting FASN. FASN expression was compared with the apoptotic index assessed by the terminal deoxynucleotidyltransferase-mediated UTP end-labeling method in 745 human prostate cancer samples by using the least squares means procedure. All statistical tests were two-sided. RESULTS: Forced expression of FASN in iPrECs, AR-iPrECs, and LNCaP cells increased cell proliferation and soft agar growth. iPrECs that expressed both FASN and androgen receptor (AR) formed invasive adenocarcinomas in immunodeficient mice (12 of 14 mice injected formed tumors vs 0 of 14 mice injected with AR-iPrEC expressing empty vector (P < .001, Fisher exact test); however, iPrECs that expressed only FASN did not. Transgenic expression of FASN in mice resulted in prostate intraepithelial neoplasia, the incidence of which increased from 10% in 8- to 16-week-old mice to 44% in mice aged 7 months or more (P = .0028, Fisher exact test), but not in invasive tumors. In LNCaP cells, siRNA-mediated silencing of FASN resulted in apoptosis. FASN overexpression protected iPrECs from apoptosis induced by camptothecin but did not protect iPrECs from Fas receptor-induced apoptosis. In human prostate cancer specimens, FASN expression was inversely associated with the apoptotic rate (mean percentage of apoptotic cells, lowest vs highest quartile of FASN expression: 2.76 vs 1.34, difference = 1.41, 95% confidence interval = 0.45 to 2.39, Ptrend = .0046). CONCLUSIONS: These observations suggest that FASN can act as a prostate cancer oncogene in the presence of AR and that FASN exerts its oncogenic effect by inhibiting the intrinsic pathway of apoptosis.en
dc.description.sponsorshipSupport for this work (to M.L.) was obtained from the Prostate Cancer Foundation, the National Cancer Institute (RO1CA131945, PO1CA89021, P50 CA90381, and CA55075), Dana Farber Cancer Institute-Novartis Drug Development Program, the Linda and Arthur Gelb Center for Translational Research, a gift from Nuclea Biomarkers to the Jimmy Fund and the Loda laboratory, and the Samuel Waxman Foundation (SPA P01-Waxman-CS01). E.P. and G.Z. are supported by a fellowship of the American?Italian Cancer Foundation.en
dc.format.extent3958-64en
dc.language.isoenen
dc.relation.ispartofseriesJournal of clinical oncology : official journal of the American Society of Clinical Oncologyen
dc.relation.ispartofseries28en
dc.relation.ispartofseries25en
dc.rightsYen
dc.subjectOncologyen
dc.subjectProstate canceren
dc.titleFatty acid synthase polymorphisms, tumor expression, body mass index, prostate cancer risk, and survival.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/finnsen
dc.identifier.rssinternalid69313en
dc.identifier.doihttp://dx.doi.org/10.1200/JCO.2009.27.0793en
dc.identifier.rssurihttp://dx.doi.org/10.1093/jnci/djp030en
dc.identifier.orcid_id0000-0002-8628-5814en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record