Show simple item record

dc.contributor.authorO'Driscoll, Lorraineen
dc.contributor.editorLewis A Chodoshen
dc.date.accessioned2010-06-08T14:52:03Z
dc.date.available2010-06-08T14:52:03Z
dc.date.issued2010en
dc.date.submitted2010en
dc.identifier.citationLewis A Chodosh, Melanoma-associated antigen family protein-D4: clinical significance and functional relevance in breast cancer, Breast Cancer Research, 12, 1, 2010, 10-11en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/40100
dc.descriptionPUBLISHEDen
dc.description.abstractMelanoma-associated antigen (MAGE) family genes are broadly expressed during development and are involved in the regulation of cell survival, cell cycle progression and apoptosis. MAGE family proteins are generally described as tumour-specific antigens and as representing ideal targets for cancer immunotherapy. In the current study, we identified melanoma-associated antigen protein-D4 (MAGE-D4), a recently characterised MAGE family member, as a new prognostic biomarker and potential therapeutic target for breast cancer. Specifically, in a whole genome microarray analysis of 103 cases of invasive breast tumours, MAGE-D4 expression was observed in 43.8% of tumours, while undetectable in normal breast tissue. Multivariate and univariate analyses also indicated MAGE-D4 expression to be associated with tumour grade, spread to lymph nodes and shortened times to relapse (P = 0.0369) and death (P = 0.0133) from time of cancer diagnosis; suggesting a role for MAGE-D4 in tumour progression. To further investigate the involvement of MAGE-D4 in breast cancer cell biology, the phenotypic effects of this gene were characterised in vitro. We observed a marked upregulation of MAGE-D4 expression - at both mRNA and protein levels - in the breast cancer cell line Hs578T compared with the syngenic Hs578Bst breast cell line. Interestingly, RNA interference-mediated knockdown of MAGE-D4 expression in Hs578T cells significantly reduced cell migration and invasion and correlated with inhibition of STAT3 and NF-?B p65 subunit phosphorylation, thus affecting two common signalling pathways involved in regulating cancer progression. Moreover, monolayer cell growth rate was not affected by MAGE-D4 gene knockdown, while growth in soft agar was significant compromised. Our results indicate that MAGE-D4 contributes to the tumorigenesis of breast cancer cells by regulating migration, invasion and anchorage-independent growth, and therefore may represent a novel target for the detection and treatment of breast cancer.en
dc.format.extent10-11en
dc.language.isoenen
dc.relation.ispartofseries12en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectCanceren
dc.subjectMelanoma-associated antigen (MAGE)en
dc.titleMelanoma-associated antigen family protein-D4: clinical significance and functional relevance in breast canceren
dc.title.alternativeBreast Cancer Researchen
dc.title.alternativeBreast Cancer Research 2010en
dc.typeProceedings of a Conferenceen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/lodriscen
dc.identifier.rssinternalid66782en
dc.subject.TCDThemeCanceren
dc.identifier.rssurihttp://hdl.handle.net/2262/40100en
dc.identifier.orcid_id0000-0002-9860-8262en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record