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dc.contributor.authorPrendergast, Patricken
dc.contributor.authorCAMPBELL, VERONICAen
dc.contributor.authorFARRELL, ERICen
dc.contributor.authorBYRNE, ELAINEen
dc.date.accessioned2010-05-19T15:17:35Z
dc.date.available2010-05-19T15:17:35Z
dc.date.issued2007en
dc.date.submitted2007en
dc.identifier.citationFarrell, E, Byrne, EM, Fischer, J, O'Brien, FJ, O'Connell, BC, Prendergast, PJ, Campbell, VA, A comparison of the osteogenic potential of adult rat mesenchymal stem cells cultured in 2-D and on 3-D collagen glycosaminoglycan scaffolds., Technology and Healthcare, 15, 1, 2007, 19 - 31en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/39604
dc.descriptionPUBLISHEDen
dc.descriptionPMID: 17264410en
dc.description.abstractAdult mesenchymal stem cells (MSCs) have the capability to differentiate along several lineages including those of bone, cartilage, tendon and muscle, thus offering huge potential for the field of tissue engineering. The purpose of this study was to characterise the differentiation capacity of rat MSCs cultured on standard plastic coverslips in 2 dimensions and on a novel collagen glycosaminoglycan scaffold in the presence of a standard combination of osteoinductive factors. Cells were cultured for 3, 7, 14 and 21 days and several markers of osteogenesis were analysed. While the initial response of the cells in 3-D seemed to be faster than cells cultured in 2-D, as evidenced by collagen type I expression, later markers showed that osteogenic differentiation of MSCs took longer in the 3-D environment of the collagen GAG scaffold compared to standard 2-D culture conditions. Furthermore, it was shown that complete scaffold mineralisation could be evoked within a 6 week timeframe. This study further demonstrates the potential use of MSC-seeded collagen GAG scaffolds for bone tissue engineering applications.en
dc.format.extent19en
dc.format.extent31en
dc.language.isoenen
dc.relation.ispartofseriesTechnology and Healthcareen
dc.relation.ispartofseries15en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectMesenchymal stem cellen
dc.subjectosteogenesisen
dc.titleA comparison of the osteogenic potential of adult rat mesenchymal stem cells cultured in 2-D and on 3-D collagen glycosaminoglycan scaffolds.en
dc.typeJournal Articleen
dc.contributor.sponsorHigher Education Authority (HEA)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pprenderen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/vacmpbllen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/byrnee2en
dc.identifier.peoplefinderurlhttp://people.tcd.ie/efarrellen
dc.identifier.rssinternalid43237en
dc.subject.TCDThemeNext Generation Medical Devicesen
dc.identifier.rssurihttp://iospress.metapress.com/content/1v0qt70uwkexjjqg/fulltext.pdfen


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