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dc.contributor.authorFARES, MARIO ALIen
dc.date.accessioned2010-02-01T14:57:10Z
dc.date.available2010-02-01T14:57:10Z
dc.date.issued2010en
dc.date.submitted2010en
dc.identifier.citationGarceau V, Smith J, Paton IR, Davey M, Fares MA, Sester DP, Burt DW, Hume DA., Pivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene products, Journal of Leukocyte Biology, 87, 2010, 753-764en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/36710
dc.descriptionPUBLISHEDen
dc.description.abstractMacrophages are involved in many aspects of development, host defense, pathology, and homeostasis. Their normal differentiation, proliferation, and survival are controlled by CSF-1 via the activation of the CSF-1R. A recently discovered cytokine, IL-34, was shown to bind the same receptor in humans. Chicken is a widely used model organism in developmental biology, but the factors that control avian myelopoiesis have not been identified previously. The CSF-1, IL-34, and CSF1R genes in chicken and zebra finch were identified from respective genomic/cDNA sequence resources. Comparative analysis of the avian CSF1R loci revealed likely orthologs of mammalian macrophage-specific promoters and enhancers, and the CSF1R gene is expressed in the developing chick embryo in a pattern consistent with macrophage-specific expression. Chicken CSF-1 and IL-34 were expressed in HEK293 cells and shown to elicit macrophage growth from chicken BM cells in culture. Comparative sequence and co-evolution analysis across all vertebrates suggests that the two ligands interact with distinct regions of the CSF1R. These studies demonstrate that there are two separate ligands for a functional CSF1R across all vertebrates.en
dc.format.extent753-764en
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.relation.ispartofseriesJournal of Leukocyte Biologyen
dc.relation.ispartofseries87en
dc.rightsYen
dc.subjectChickenen
dc.subjectzebra finchen
dc.subjectmacrophageen
dc.subjectco-evolutionen
dc.titlePivotal Advance: Avian colony-stimulating factor 1 (CSF-1), interleukin-34 (IL-34), and CSF-1 receptor genes and gene productsen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/faresmen
dc.identifier.rssinternalid63301en
dc.identifier.rssurihttp://dx.doi.org/10.1189/jlb.0909624
dc.identifier.rssurihttp://dx.doi.org/10.1189/jlb.0909624en


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