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dc.contributor.authorMILLS, KINGSTONen
dc.contributor.authorMCQUILLAN, KEITHen
dc.contributor.authorLYNCH, MARINAen
dc.date.accessioned2010-01-18T13:19:02Z
dc.date.available2010-01-18T13:19:02Z
dc.date.issued2010en
dc.date.submitted2010en
dc.identifier.citationMc Quillan, KL, Lynch MA, and Mills KH., Activation of mixed glia by A-beta-specific Th1 and Th17 cells and its regulation by Th2 cells, Brain, Behavior, and Immunity, 24, 4, 2010, 598-607en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/35748
dc.descriptionPUBLISHEDen
dc.description.abstractMicroglia are innate immune cells of the CNS, that act as antigen-presenting cells (APC) for antigen-specific T cells and respond to inflammatory stimuli, such as amyloid-beta (A?), resulting in the release of neurotoxic factors and pro-inflammatory cytokines. Astrocytes can also act as APC and modulate the function of microglia. However, the role of distinct T cell subtypes, in particular Th17 cells, in glial activation and subsequent modulatory effects of Th2 cells are poorly understood. Here, we generated A?-specific Th1, Th2, and Th17 cells and examined their role in modulating A?-induced activation of microglia in a mixed glial culture, a preparation which mimics the complex APC types in the brain. We demonstrated that mixed glia acted as an effective APC for A?-specific Th1 and Th17 cells. Addition of A?-specific Th2 cells suppressed the A?-induced IFN-? production by Th1 cells and IL-17 production by Th17 cells with glia as the APC. Co-culture of A?-specific Th1 or Th17 cells with glia markedly enhanced A?-induced pro-inflammatory cytokine production and expression of MHC class II and co-stimulatory molecules on the microglia. Addition of A?-specific Th2 cells inhibited Th17 cell-induced IL-1? and IL-6 production by mixed glia and attenuated Th1 cell-induced CD86 and CD40 expression on microglia. The modest enhancement of MHC class II and CD86 expression on astrocytes by A?-specific Th1 and Th17 was not attenuated by Th2 cells. These data indicate that A?-specific Th1 and Th17 cells induce inflammatory activation of glia, and that this is in part regulated by Th2 cells.en
dc.description.sponsorshipScience Foundation Irelanden
dc.format.extent598-607en
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.relation.ispartofseriesBrain, Behavior, and Immunityen
dc.relation.ispartofseries24en
dc.relation.ispartofseries4en
dc.rightsYen
dc.subjectMicrogliaen
dc.subjectamyloid-betaen
dc.subjectTh1en
dc.subjectTh2en
dc.subjectTh17 cellen
dc.titleActivation of mixed glia by A-beta-specific Th1 and Th17 cells and its regulation by Th2 cellsen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/millsken
dc.identifier.peoplefinderurlhttp://people.tcd.ie/lynchmaen
dc.identifier.rssinternalid63058en
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDThemeNeuroscienceen
dc.identifier.rssurihttp://dx.doi.org.elib.tcd.ie/10.1016/j.bbi.2010.01.003en
dc.identifier.rssurihttp://dx.doi.org/10.1016/j.bbi.2010.01.003


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