dc.contributor.author | KELLEHER, DERMOT P | |
dc.date.accessioned | 2009-10-23T16:24:38Z | |
dc.date.available | 2009-10-23T16:24:38Z | |
dc.date.issued | 1996 | |
dc.date.submitted | 1996 | en |
dc.identifier.citation | X. G. Fan, D. Kelleher, X. J. Fan, H. X. Xia and P. W. Keeling `Helicobacter pylori increases proliferation of gastric epithelial cells? in Gut, 38, (1), 1996, pp 19-22 | en |
dc.identifier.other | Y | en |
dc.identifier.other | Y | |
dc.identifier.other | 21329 | |
dc.identifier.uri | http://hdl.handle.net/2262/34195 | |
dc.description | PUBLISHED | en |
dc.description.abstract | The direct and indirect effects of helicobacter pylori on cell kinetics of gastric epithelial cell line AGS were investigated by flow cytometric analysis of Ki-67 positive cells and by MTT assay. Flow cytometric analysis of Ki-67 positivity permits detection of cells that are in S-phase, whereas the MTT assay is a colometric measure of the number of viable cells. In the absence of added stimulants, 23.06 (4.88)% mean (SD) of AGS cells were Ki-67 positive. When cells were preincubated in the presence of H pylori, there was a significant increase in Ki-67 positivity (66.20 (7.89)%, p < 0.001). This increase was not seen in cells cultured in the presence of Campylobacter jejuni (24.63 (8.11)% or Escherichia coli (21.66 (9.78)%). Pre-incubation of AGS cells with supernatants from both H pylori and mitogen activated peripheral blood lymphocytes also increased the per cent of cells that were Ki-67 positive (72.93 (8.68) and 69.96 (12.35)%; p, 0.001) respectively. Similar results were also found in MTT assay. These data show that both H pylori directly and the immune/inflammatory response to H pylori indirectly can influence the rate of epithelial cell proliferation, suggesting this bacterium may be an initiating step in gastric carcinogenesis and an important co-carcinogenic factor in H pylori positive subjects. | en |
dc.format.extent | 19-22 | en |
dc.format.extent | 1050609 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.publisher | BMJ | en |
dc.relation.ispartofseries | Gut | en |
dc.relation.ispartofseries | 38 | en |
dc.relation.ispartofseries | 1 | en |
dc.rights | Y | en |
dc.subject | Gastroenterology | |
dc.subject | Hepatology | |
dc.title | Helicobacter pylori increases proliferation of gastric epithelial cells | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kellehdp | |
dc.identifier.rssinternalid | 21001 | |