Show simple item record

dc.contributor.authorMc Manus, Rossen
dc.contributor.authorKelleher, Dermoten
dc.contributor.authorWindle, Henryen
dc.date.accessioned2009-10-07T18:53:12Z
dc.date.available2009-10-07T18:53:12Z
dc.date.issued2005en
dc.date.submitted2005en
dc.identifier.citationWindle HJ*, Ang YS*, Athie Morales V, McManus R*, Kelleher DP, Human peripheral and gastric lymphocyte responses to Helicobacter pylori NapA and AphC differ in infected and uninfected individuals, Gut, 54, 1, 2005, 25 - 32en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/33845
dc.descriptionPUBLISHEDen
dc.description(* These authors contributed equally) PMID: 15591500en
dc.description.abstractBACKGROUND: In this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (PBMC) and lamina propria gastric lymphocytes (LPL) to two Helicobacter pylori antigens, the neutrophil activating protein (NapA) and alkyl hydroperoxide reductase (AphC). These antigens were identified and selected for study based on the observation that serological recognition of these proteins was associated with H pylori negative status in humans. AIMS: The aim was to study the serological, proliferative, and cytokine responses of PBMC and LPL, obtained from H pylori infected and uninfected individuals, to these antigens. METHODS: Patient serum, PBMC, and LPL were used to determine antibody isotype, and proliferative and cytokine responses to recombinant forms of NapA and AphC using western blotting and ELISA. RESULTS: Western blotting revealed antibody reactivity to recombinant NapA and AphC among the H pylori negative population studied. Both the proliferative and interferon gamma responses of PBMC and LPL to NapA and AphC were significantly higher in H pylori negative compared with H pylori positive subjects. Analysis of the IgG subclass profiles to both antigens revealed a T helper 1 associated IgG3 antibody response in uninfected individuals. However, interleukin 10 production was greater in H pylori positive individuals in response to these antigens. CONCLUSIONS: Taken together these data are consistent with an immune response to these antigens skewed towards a T helper 1 response in the uninfected cohort.en
dc.description.sponsorshipThis work was supported by a Health Research Board grant (YSA).en
dc.format.extent25en
dc.format.extent32en
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.relation.ispartofseriesGuten
dc.relation.ispartofseries54en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectClinical Medicineen
dc.titleHuman peripheral and gastric lymphocyte responses to Helicobacter pylori NapA and AphC differ in infected and uninfected individualsen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/rmcmanusen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/hjwindleen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kellehdpen
dc.identifier.rssinternalid11635en
dc.identifier.doihttp://dx.doi.org/10.1136/gut.2003.025494en
dc.subject.TCDThemeCanceren
dc.subject.TCDThemeImmunology, Inflammation & Infectionen
dc.subject.TCDThemeInternational Developmenten
dc.identifier.rssurihttp://dx.doi.org/10.1136/gut.2003.025494


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record