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dc.contributor.authorKELLEHER, DERMOT P
dc.date.accessioned2009-10-07T18:46:29Z
dc.date.available2009-10-07T18:46:29Z
dc.date.issued2001
dc.date.submitted2001en
dc.identifier.citationH. M. Roche, A. M. Terres, I. B. Black, M. J. Gibney and D. Kelleher `Fatty acids and epithelial permeability: effect of conjugated linoleic acid in Caco-2 cells? in Gut, 48, (6), 2001, pp 797-802en
dc.identifier.otherYen
dc.identifier.otherY
dc.identifier.other21324
dc.identifier.urihttp://hdl.handle.net/2262/33832
dc.descriptionPUBLISHEDen
dc.description.abstractConjugated linoleic acid (CLA) is a collective term referring to the positional and geometric isomers of linoleic acid. This novel fatty acid has been shown to have a number of beneficial actions, including immunomodulatory, anticarcinogenic, and antiatherogenic effects. Tight junctions of epithelial cells determine epithelial membrane integrity and selective paracellular permeability to ions and macromolecules. Occludin and ZO-1 are integral structural components of the tight junction, which are involved in the biogenesis and functional integrity of the epithelial monolayer. This study investigated the effects of two isomers of CLA (cis-9 and trans-10 isomers) on Caco-2 cell transepithelial resistance (TER) development, paracellular epithelial permeability, and occludin and ZO-1 expression. Caco-2 cells were grown in media supplemented with 0.05 mM linoleic acid, cis-9 CLA, or trans-10 CLA for 21 days. The trans-10 CLA isomer delayed Caco-2 cell TER development, which is an in vitro measure of epithelial cell integrity, and increased paracellular epithelial permeability. Immunofluorescent staining of Caco-2 cell epithelial monolayers grown in media supplemented trans-10 CLA showed that the trans-10 CLA isomer altered distribution of occludin and ZO-1. The trans-10 CLA isomer delayed the acquisition of transepithelial resistance and altered the cellular distribution of occludin, which have important implications in relation to epithelial permeability.en
dc.format.extent797-802en
dc.format.extent238866 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherBMJen
dc.relation.ispartofseriesGuten
dc.relation.ispartofseries48en
dc.relation.ispartofseries6en
dc.rightsYen
dc.subjectconjugated linoleic acid; occludin; ZO-1; tight junction; epithelial permeability; Caco-2 cellsen
dc.titleFatty acids and epithelial permeability: effect of conjugated linoleic acid in Caco-2 cellsen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kellehdp
dc.identifier.rssinternalid568
dc.identifier.rssurihttp://dx.doi.org/10.1136/gut.48.6.797


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