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dc.contributor.authorO'NEILL, LUKE ANTHONY JOHN
dc.date.accessioned2009-10-05T15:01:25Z
dc.date.available2009-10-05T15:01:25Z
dc.date.issued1992
dc.date.submitted1992en
dc.identifier.citationStylianou E, O'Neill LA, Rawlinson L, Edbrooke MR, Woo P, Saklatvala J. `Interleukin 1 induces NF-kappa B through its type I but not its type II receptor in lymphocytes? in Journla of Biological Chemistry, 267, (22), 1992, pp 15836 - 15841en
dc.identifier.otherY
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/33729
dc.descriptionPUBLISHEDen
dc.description.abstractIt is not known whether one or both of the interleukin 1 (IL1) receptors mediates the induction of the DNA-binding protein NF-kappa B. Nuclear extracts of the murine lines EL4.NOB.1 and 70Z/3, which bear the type I (80 kDa) and type II (67 kDa) IL1 receptor, respectively, were analyzed by an electrophoretic mobility shift assay. A 265-base pair sequence of the human serum amyloid A gene or a synthetic oligonucleotide each containing the NF-kappa B site were used as the DNA probes. IL1 induction of NF-kappa B was rapid (optimal at 15-30 min) and transient in both cell types. The IL1 receptor antagonist (IL1ra), which binds strongly to the type I receptor, inhibited the NF-kappa B response in both cell lines. IL1ra did not bind to the type II receptor on 70Z/3 cells as judged by competition for binding with 125I-IL1 alpha. When 125I-IL1ra binding to 70Z/3 cells was measured, a small number (10/cell) of high affinity sites (Kd = 5 x 10(-12) M) were detected. These were likely to have been type I receptor because an antibody to this inhibited the NF-kappa B induction in 70Z/3 cells (as well as EL4). Potential signal transduction mechanisms involving protein kinase C or oxygen radicals were studied. Phorbol 12-myristate 13-acetate induced NF-kappa B with a similar time course to IL1 in 70Z/3 but only after 4 h in EL4.IL1 was unaffected by a protein kinase C inhibitor (staurosporine). H2O2 did not mimic IL1, and IL1 was not inhibited by an antioxidant. The type I receptor mediates the induction of NF-kappa B in response to IL1 via a signaling mechanism that still remains to be identified.en
dc.format.extent15836en
dc.format.extent15841en
dc.format.extent5310158 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherThe American Society for Biochemistry and Molecular Biologyen
dc.relation.ispartofseriesJournla of Biological Chemistryen
dc.relation.ispartofseries267en
dc.relation.ispartofseries22en
dc.rightsYen
dc.subjectBiochemistryen
dc.titleInterleukin 1 induces NF-kappa B through its type I but not its type II receptor in lymphocytes.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/laoneill
dc.identifier.rssinternalid5021


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