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dc.contributor.authorO'NEILL, LUKE ANTHONY JOHN
dc.date.accessioned2009-09-30T21:53:25Z
dc.date.available2009-09-30T21:53:25Z
dc.date.issued2003
dc.date.submitted2003en
dc.identifier.citationC. Wietek, S. M. Miggin, C. A. Jefferies and L. A. O'Neill `Interferon regulatory factor-3-mediated activation of the interferon-sensitive response element by Toll-like receptor (TLR) 4 but not TLR3 requires the p65 subunit of NF-kappa? in The Journal of Biological Chemistry, 278, (51), 2003, pp 50923-50931en
dc.identifier.otherY
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/33691
dc.descriptionPUBLISHEDen
dc.description.abstractInterferon regulatory factor (IRF) 3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptor 3 (TLR3) and TLR4. We have found that a dominant negative form of I?B kinase 2 and a mutant form of I?B, which acts as a super-repressor of NF-?B, blocked activation of the ISRE by the TLR4 ligand lipopolysaccharide but not the TLR3 ligand poly(I-C). TLR4 failed to activate the ISRE in mouse embryonic fibroblasts bearing a targeted deletion of p65, whereas the response to TLR3 in these cells was normal. The p65 subunit of NF-?B was detected in the lipopolysaccharide-activated but not poly(I-C)-activated ISRE-binding complex. Finally, p65 promoted transactivation of gene expression by IRF-3. These results therefore indicate that IRF-3-mediated activation of the ISRE by TLR4 but not TLR3 requires the p65 subunit of NF-?B.en
dc.description.sponsorshipThis work was supported by the Science Foundation Ireland.en
dc.format.extent50923-50931en
dc.format.extent451863 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherThe American Society for Biochemistry and Molecular Biologyen
dc.relation.ispartofseriesThe Journal of Biological Chemistryen
dc.relation.ispartofseries278en
dc.relation.ispartofseries51en
dc.rightsYen
dc.subjectBiochemistryen
dc.titleInterferon regulatory factor-3-mediated activation of the interferon-sensitive response element by Toll-like receptor (TLR) 4 but not TLR3 requires the p65 subunit of NF-kappaen
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/laoneill
dc.identifier.rssinternalid20829
dc.identifier.rssurihttp://dx.doi.org/10.1074/jbc.M308135200


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