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dc.contributor.authorMILLS, KINGSTON
dc.date.accessioned2009-09-28T18:43:38Z
dc.date.available2009-09-28T18:43:38Z
dc.date.issued1988
dc.date.submitted1988en
dc.identifier.citationMills, K.H.G. `Inhibitory effects of monoclonal antibodies to a synthetic peptide of influenza haemagglutinin on the processing and presentation of viral antigens to class II-restricted T cell clones? in Immunology, 65, 1988, pp 365 - 371en
dc.identifier.otherY
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/33385
dc.descriptionPUBLISHEDen
dc.description.abstractMonoclonal antibodies (mAb) prepared against a synthetic peptide of influenza virus haemagglutinin (HA), containing a known T-cell determinant, were used to examine the mechanism of antigen-induced activation of HA-specific class II-restricted T-cell clones. Previous studies had shown that T-cell clones, established from mice primed by infection with influenza virus, recognize variable antibody binding region of HA, including a determinant formed from residues within the sequence HA1 48-68. MAb to the synthetic peptide, p48-68, recognized purified HA and whole virus in an ELISA, and their specificity pattern for natural variant viruses was similar to that described for the T-cell clones specific for the same peptide. The anti-peptide mAb inhibited peptide or virus-induced proliferation of the peptide specific T-cell clones (but has no effect on a unrelated HA-specific clone), whereas mAb to the native HA molecule inhibited virus but not peptide-induced T-cell activation. In addition, the anti-peptide mAb showed significant inhibition of T-cell proliferation to peptide or virus pulsed antigen-presenting cell (APC). The results suggest that the anti-HA mAb affect antigen induced T-cell activation simply through blocking virus uptake by the APC, whereas the anti-peptide antibodies, which appear to recognize the same determinant on the peptide and the processed antigen, mediate their effect at the level of antigen presentation.en
dc.format.extent365en
dc.format.extent371en
dc.format.extent1125434 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherBritish Society for Immunologyen
dc.relation.ispartofseriesImmunologyen
dc.relation.ispartofseries65en
dc.rightsYen
dc.subjectBiochemistryen
dc.titleInhibitory effects of monoclonal antibodies to a synthetic peptide of influenza haemagglutinin on the processing and presentation of viral antigens to class II-restricted T cell clonesen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/millsk


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