dc.contributor.author | LAVELLE, EDWARD | |
dc.contributor.author | MILLS, KINGSTON | |
dc.date.accessioned | 2009-09-28T13:16:04Z | |
dc.date.available | 2009-09-28T13:16:04Z | |
dc.date.issued | 2003 | |
dc.date.submitted | 2003 | en |
dc.identifier.citation | Lavelle, E., McNeela, E., Armstrong, M., Leavy, O., Higgins, S.C. and Mills K.H.G. `Cholera toxin promotes the induction of regulatory T cells by modulation dendritic cell activation? in The Journal of Immunology, 171, 2003, pp 2384 - 2392 | en |
dc.identifier.other | Y | |
dc.identifier.other | Y | en |
dc.identifier.uri | http://hdl.handle.net/2262/33330 | |
dc.description | PUBLISHED | en |
dc.description.abstract | It has previously been reported that cholera toxin (CT) is a potent mucosal adjuvant that enhances Th2 or mixed Th1/Th2 type responses to coadministered foreign Ag. Here we demonstrate that CT also promotes the generation of regulatory T (Tr) cells against bystander Ag. Parenteral immunization of mice with Ag in the presence of CT induced T cells that secreted high levels of IL-4 and IL-10 and lower levels of IL-5 and IFN-. Ag-specific CD4+ T cell lines and clones generated from these mice had cytokine profiles characteristic of Th2 or type 1 Tr cells, and these T cells suppressed IFN- production by Th1 cells. Furthermore, adoptive transfer of bone marrow-derived dendritic cells (DC) incubated with Ag and CT induced T cells that secreted IL-4 and IL-10 and low concentrations of IL-5. It has previously been shown that IL-10 promotes the differentiation or expansion of type 1 Tr cells. Here we found that CT synergized with low doses of LPS to induce IL-10 production by immature DC. CT also enhanced the expression of CD80, CD86, and OX40 (CD134) on DC and induced the secretion of the chemokine, macrophage inflammatory protein-2 (MIP-2), but inhibited LPS-driven induction of CD40 and ICAM-I expression and production of the inflammatory cytokines/chemokines IL-12, TNF-, MIP-1, MIP-1, and monocyte chemoattractant protein-1. Our findings suggest that CT induces maturation of DC, but, by inducing IL-10, inhibiting IL-12, and selectively affecting surface marker expression, suppresses the generation of Th1 cells and promotes the induction of T cells with regulatory activity. | en |
dc.description.sponsorship | This work was supported by funds from the Science Foundation Ireland and the European Union (EU) 5th Framework (Contract QLK2 CT 1999 00228) | en |
dc.format.extent | 2384 | en |
dc.format.extent | 2392 | en |
dc.format.extent | 330145 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.publisher | The American Association of Immunologists | en |
dc.relation.ispartofseries | The Journal of Immunology | en |
dc.relation.ispartofseries | 171 | en |
dc.rights | Y | en |
dc.subject | Biochemistry | en |
dc.title | Cholera toxin promotes the induction of regulatory T cells by modulation dendritic cell activation | en |
dc.type | Journal Article | en |
dc.contributor.sponsor | Science Foundation Ireland | |
dc.contributor.sponsor | European Union (EU) | |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/millsk | |
dc.identifier.rssinternalid | 7755 | |