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dc.contributor.authorKELLY, DANIEL
dc.contributor.authorPrendergast, Patrick John
dc.date.accessioned2009-04-14T17:07:43Z
dc.date.available2009-04-14T17:07:43Z
dc.date.issued2007
dc.date.submitted2007en
dc.identifier.citationD.J. Kelly, A. Crawford, S.C. Dickinson, T.J. Sims, A.P. Hollander, P.J. Prendergast and P.V. Hatton `Biochemical markers of the mechanical quality of engineered hyaline cartilage? in Journal of Materials Science: Materials in Medicine, 18, (2), 2007, pp 273-281en
dc.identifier.otherYen
dc.identifier.otherY
dc.identifier.urihttp://hdl.handle.net/2262/29146
dc.descriptionPUBLISHEDen
dc.description.abstractThe aim of this study was to determine whether or not biochemical markers can be used as surrogate measures for the mechanical quality of tissue engineered cartilage. The biochemical composition of tissue engineered cartilage constructs were altered by varying either (i) the initial cell seeding density of the scaffold (seeding density protocol) or (ii) the length of time the engineered tissue was cultured (culture period protocol). The aggregate or Young?s moduli of the constructs were measured (by confined or unconfined compression respectively), and compared with the composition of the extracellular matrix by quantitative measurement of the glycosaminoglycan (GAG), hydroxyproline, collagen I and collagen II and collagen cross-links. The aggregate modulus correlated positively with both GAG and collagen II content, but not with collagen I content. Young?s modulus correlated positively with GAG, collagen II and collagen I content, and the ratio of mature to immature cross-links, but not with hydroxyproline content. These results suggested that hydroxyproline may be an unreliable indicator of mechanical quality of tissue engineered cartilage, and that a measure of collagen II and GAG content is required to predict the biomechanical quality of tissue engineered cartilage.en
dc.description.sponsorshipThis work was funded by the European Union (EU) under the SCAFCART project (contract G5RD-CT-1999-00050). APH is funded in part by an endowed chair from the UK Arthritis Research Campaign.en
dc.format.extent273-281en
dc.format.extent458008 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherSpringeren
dc.relation.ispartofseriesJournal of Materials Science: Materials in Medicineen
dc.relation.ispartofseries18en
dc.relation.ispartofseries2en
dc.rightsYen
dc.subjectAdministrative Staff Authorsen
dc.titleBiochemical Markers of the Mechanical Quality of Engineered Hyaline Cartilageen
dc.typeJournal Articleen
dc.contributor.sponsorArthritis Research Campaign
dc.contributor.sponsorEuropean Union (EU)
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pprender
dc.identifier.rssurihttp://dx.doi.org/10.1007/s10856-006-0689-2


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