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dc.contributor.authorKELLY, DANIELen
dc.date.accessioned2009-04-14T16:27:19Z
dc.date.available2009-04-14T16:27:19Z
dc.date.issued2009en
dc.date.submitted2009en
dc.identifier.citationJungreuthmayer, C., Jaasma, M.J., Al-Munajjed, A.A., Zanghellini, J., Kelly, D.J., O'Brien, F.J., Deformation simulation of cells seeded on a collagen-GAG scaffold in a flow perfusion bioreactor using a sequential 3D CFD-elastostatics model., Medical Engineering and Physics, 31, 4, 2009, 420-427en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/29107
dc.descriptionPUBLISHEDen
dc.descriptionPMID: 19109048en
dc.description.abstractTissue-engineered bone shows promise in meeting the huge demand for bone grafts caused by up to 4 million bone replacement procedures per year, worldwide. State-of-the-art bone tissue engineering strategies use flow perfusion bioreactors to apply biophysical stimuli to cells seeded on scaffolds and to grow tissue suitable for implantation into the patient's body. The aim of this study was to quantify the deformation of cells seeded on a collagen-GAG scaffold which was perfused by culture medium inside a flow perfusion bioreactor. Using a ?CT scan of an unseeded collagen-GAG scaffold, a sequential 3D CFD-deformation model was developed. The wall shear stress and the hydrostatic wall pressure acting on the cells were computed through the use of a CFD simulation and fed into a linear elastostatics model in order to calculate the deformation of the cells. The model used numerically seeded cells of two common morphologies where cells are either attached flatly on the scaffold wall or bridging two struts of the scaffold. Our study showed that the displacement of the cells is primarily determined by the cell morphology. Although cells of both attachment profiles were subjected to the same mechanical load, cells bridging two struts experienced a deformation up to 500 times higher than cells only attached to one strut. As the scaffold's pore size determines both the mechanical load and the type of attachment, the design of an optimal scaffold must take into account the interplay of these two features and requires a design process that optimizes both parameters at the same time.en
dc.description.sponsorshipWe are very grateful to Dr. Alex Lennon for his technical support and assistance with the Linux computer cluster. This project was funded by a Science Foundation Ireland Industry Research Partnership with Siemens.en
dc.format.extent420-427en
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.relation.ispartofseriesMedical Engineering and Physicsen
dc.relation.ispartofseries31en
dc.relation.ispartofseries4en
dc.rightsYen
dc.subjectMechanical & Manufacturing Engineeringen
dc.titleDeformation simulation of cells seeded on a collagen-GAG scaffold in a flow perfusion bioreactor using a sequential 3D CFD-elastostatics model.en
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kellyd9en
dc.identifier.rssinternalid56663en
dc.identifier.rssurihttp://dx.doi.org/10.1016/j.medengphy.2008.11.003
dc.identifier.rssurihttp://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T9K-4V6RNTF-2-R&_cdi=5117&_user=103681&_orig=search&_coverDate=05%2F31%2F2009&_sk=999689995&view=c&wchp=dGLbVtz-zSkWb&md5=6b704f588e0e3c6f41a2bcb4088cfcdc&ie=/sdarticle.pdfen


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