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dc.contributor.authorVINARDELL, TATIANAen
dc.contributor.authorKELLY, DANIELen
dc.contributor.authorCAMPBELL, VERONICA ANNen
dc.contributor.authorBUCKLEY, CONOR TIMOTHYen
dc.date.accessioned2009-04-14T12:14:16Z
dc.date.available2009-04-14T12:14:16Z
dc.date.issued2008en
dc.date.submitted2008en
dc.identifier.citationThorpe, S. D., Buckley, C. T., Vinardell, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J., Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells., Biochemical and Biophysical Research Communications, 337, 2, 2008, 458-462en
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/29030
dc.descriptionPUBLISHEDen
dc.descriptionPMID: 18851955en
dc.description.abstractThe objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-?3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5 ? 0.21%w/w vs. 0.94 ? 0.03%w/w) and annulus (1.09 ? 0.09%w/w vs. 0.59 ? 0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue.en
dc.description.sponsorshipFunding was provided by Science Foundation Ireland (07-RFP- ENMF142) and Enterprise Ireland (PC / 2006/ 384).en
dc.format.extent458-462en
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.relation.ispartofseriesBiochemical and Biophysical Research Communicationsen
dc.relation.ispartofseries337en
dc.relation.ispartofseries2en
dc.rightsYen
dc.subjectChondrogenesisen
dc.subjectMesenchymal stem cellsen
dc.subjectTGF-?3en
dc.subjectDynamic compressionen
dc.subjectTissue engineeringen
dc.titleDynamic compression can inhibit chondrogenesis of mesenchymal stem cells.en
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorEnterprise Ireland
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kellyd9en
dc.identifier.peoplefinderurlhttp://people.tcd.ie/vinardten
dc.identifier.peoplefinderurlhttp://people.tcd.ie/vacmpbllen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/cbuckleen
dc.identifier.rssinternalid56667en
dc.identifier.rssurihttp://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-4TN5GCK-B-7&_cdi=6713&_user=103681&_orig=search&_coverDate=12%2F12%2F2008&_sk=996229997&view=c&wchp=dGLbVzz-zSkzk&md5=9ebf2b016004cf9a1340007c7f7616dc&ie=/sdarticle.pdfen
dc.identifier.rssurihttp://dx.doi.org/10.1016/j.bbrc.2008.09.154


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