Show simple item record

dc.contributor.authorMURPHY, PAULA
dc.date.accessioned2008-07-17T08:40:03Z
dc.date.available2008-07-17T08:40:03Z
dc.date.issued2008
dc.date.submitted2008en
dc.identifier.citationKristen Summerhurst, Margaret Stark, James Sharpe, Duncan Davidson and Paula Murphy, '3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts 19)' in Gene Expression Patterns, 8, (5), 2008, p331 - 348en
dc.identifier.issn49204
dc.identifier.otherY
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/18839
dc.descriptionPUBLISHEDen
dc.description.abstractWnt signalling is one of the fundamental cell communication systems operating in the embryo and the collection of 19 Wnt and 10 Frizzled (Fzd) receptor genes (in mouse and human) represent just part of a complex system to be unravelled. Here we present a spatially comprehensive set of data on the 3D distribution of Wnt and Fzd gene expression patterns at a carefully selected single stage of mouse development. Overviews and selected features of the patterns are presented and the full 3D data set, generated by fully described probes, is available to the research community through the Edinburgh Mouse Atlas of Gene Expression. In addition to being comprehensive, the data set has been generated and recorded in a consistent manner to facilitate comparisons between gene expression patterns with the capacity to generate matching virtual sections from the 3D representations for specific studies. Expression patterns in the left forelimb were selected for more detailed comparative description. In addition to confirming the previously published expression of these genes, our whole embryo and limb bud analyses significantly extend the data in terms of details of the patterns and the addition of previously undetected sites of expression. Our focussed analysis of expression domains in the limb, defined by just two gene families, reveals a surprisingly high degree of spatial complexity and underlines the enormous potential for local cellular interactions that exist within an emerging structure. This work also highlights the use of OPT to generate detailed high-quality, spatially complex expression data that is readily comparable between specimens and can be reviewed and reanalysed as required for specific studies. It represents a core set of data that will be extended with additional stages of development and through addition of potentially interacting genes and ultimately other cross-regulatory communication pathways operating in the embryo.en
dc.description.sponsorshipThis work was supported entirely by Science Foundation Ireland (Programme Award 02/IN1/B267)en
dc.format.extent331en
dc.format.extent348en
dc.format.extent2510599 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherElsevieren
dc.relation.ispartofseriesGene Expression Patternsen
dc.relation.ispartofseries8 (5) 2008en
dc.rightsYen
dc.subjectWnten
dc.subjectFzden
dc.subjectOPTen
dc.subjectMouse embryoen
dc.subject3D expression patternsen
dc.subjectCell communication systemsen
dc.title3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts 19).en
dc.typeJournal Articleen
dc.contributor.sponsorScience Foundation Ireland
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/pmurphy3
dc.identifier.rssurihttp://dx.doi.org/10.1016/j.gep.2008.01.007


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record