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dc.contributor.authorBELL, ANGUS
dc.date.accessioned2008-07-04T13:08:19Z
dc.date.available2008-07-04T13:08:19Z
dc.date.issued2003
dc.date.submitted2003en
dc.identifier.citationMitchell, D.P. and Bell, A. 'PEST sequences in the malaria parasite Plasmodium falciparum: a genomic study' in Malaria Journal, 2, (1), 2003.en
dc.identifier.issn5541
dc.identifier.otherY
dc.identifier.otherYen
dc.identifier.urihttp://hdl.handle.net/2262/18019
dc.descriptionPUBLISHEDen
dc.description.abstractAnopheles gambiae is the main vector of Plasmodium falciparum in Africa. The mosquito midgut constitutes a barrier that the parasite must cross if it is to develop and be transmitted. Despite the central role of the mosquito midgut in the host/parasite interaction, little is known about its protein composition. Characterisation of An. gambiae midgut proteins may identify the proteins that render An. gambiae receptive to the malaria parasite. We carried out two-dimensional gel electrophoresis of An. gambiae midgut proteins and compared protein profiles for midguts from males, sugar-fed females and females fed on human blood. Very few differences were detected between male and female mosquitoes for the approximately 375 silver-stained proteins. Male midguts contained ten proteins not detected in sugar-fed or blood-fed females, which are therefore probably involved in male-specific functions; conversely, female midguts contained twenty-three proteins absent from male midguts. Eight of these proteins were specific to sugar-fed females, and another ten, to blood-fed females. Mass spectrometry analysis of the proteins found only in blood-fed female midguts, together with data from the recent sequencing of the An. gambiae genome, should make it possible to determine the role of these proteins in blood digestion or parasite receptivity.en
dc.description.sponsorshipWe would like to thank Jean-Claude Jacques for technical assistance and Nadia Monnier for rearing mosquitoes. This work was supported in part by fellowships awarded to G.P. (MRE, FRM and CRG) and research funds from the Pasteur Institute and the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR).en
dc.format.extent16 [5 pp]en
dc.format.extent380775 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.ispartofseries2en
dc.relation.ispartofseries1en
dc.rightsYen
dc.subjectmalariaen
dc.subjectPESTen
dc.subjectcalpainen
dc.titlePEST sequences in the malaria parasite Plasmodium falciparum: a genomic studyen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/abell
dc.identifier.rssinternalid5541
dc.identifier.rssurihttp://www.malariajournal.com/content/2/1/16
dc.identifier.rssuridoi:10.1186/1475-2875-2-16


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